Diagnosis of Drug Allergy by the Lymphocyte Stimulation Test with the MTT (3-(4,5-Dimethyl thiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide) Assay.
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چکیده
منابع مشابه
Diagnosis of drug allergy by the lymphocyte stimulation test with the MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay.
The lymphocyte stimulation test (LST) is one of the most useful laboratory tests for the identification of allergy to a specific drug. The present study was conducted to examine utility of the LST using the [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay as a method for diagnosing drug allergy. The basic experimental conditions for the MTT assay were determined by u...
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The tetrazolium dye, 3-(4,5-dimethylthia/ol-2-yl)-2,5-diphi-iiyltclrazolium bromide (MTT), is reduced by live but not dead cells, and this reaction is used as the end point in a rapid drug-screening assay. It can also be used for accurate determinations of drug sensitivity but only if a quantitative relationship is established between cell number and M l'I forma/an production. We have shown tha...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 1993
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.16.686